ORF information
ORF ID TF00812 (frame 1) Gene ID lpp2740
Position and sequences 3121606...3124713(1035 aa) Gene Name/synonym dotA -
Organism Legionella pneumophila Paris Cluster 3 genes
Product Type IV secretion system protein DotA
1stcategory I-T4SS 2ndcategory DotA Family
3rdcategory Conjugation and Effector translocator
Function Component of the type IV secretion system Dot/Icm that is required for intracellular growth in human macrophages and amoebae. The DotA polytopic membrane protein is essential for function of the Dot/Icm T4SS conjugation. DotA along with an additional substrate, RalF have been shown to be involved in regulation of phagosome biogenesis and transport in Legionella.traY and dotA genes were required for R64 conjugation and RSF1010 mobilization in L. pneumophila respectively.
Miscellaneus 3rd Category: Effector translocator and Conjugation; Transmembrane: 33-50; 61-81; 101-133; 588-608; 644-670; 676-701; 708-727; 778-805; 893-913; This protein is homologous to TraY of plasmid R64, as well as a protein from Xanthomonas axonopodis. Sequence similarity between R64 TraY and L. pneumophila DotA is localized within the respective transmembrane domains.
References PubMed:9009315  PubMed:11689436  PubMed:17101653  PubMed:17040490  PubMed:15271914  PubMed:15003709  PubMed:14715899  PubMed:12081952  PubMed:10225863  PubMed:9488381  PubMed:9465074 

Functional Annotation
  KEGG database
Organism L.pneumophila_Paris Gene entry lpp2740
Gene name dotA Synonyms
Definition defect in organelle trafficking protein DotA
Classification Unassigned
DBLinks GeneID 3117985 GI 54298676 Pasteur lpp2740 UniProt Q5X1K3
 
 
COG No results found !

Blast results (KEGG)
L.pneumophila_Paris :  BlastP (output)
Score Expect Query coverage Subject coverage
2084 0.0 100 100
InterPro
 
InterPro No results found !
GO (Gene Onthology)
 
GO No results found !
Blast Results (Swiss-Prot)
 
Blast Results (Swiss-Prot) No results found !

Topology
 
PHOBIUS txt

Protein localization analysis
 
Psort (output)